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Ficoll-Paque PREMIUM

Ficoll-Paque PREMIUM 

  • 17-5442-02  6X100ml 1.078 g/ml

Ficoll-Paque PREMIUM產品系列為無菌即用型密度梯度介質，用于制備單核細胞。

Ficoll-Paque PREMIUM products are a range of sterile, ready-to-use density gradient media for the preparation of mononuclear cells.

  • Sterile, ready to use reagents used to prepare mononuclear cell preparations from peripheral blood, bone marrow, and umbilical cord blood by density gradient centrifugation.
  • Manufactured under a Quality Management System certified to ISO 13485:2012.
  • Low levels of endotoxin (< 0.12 EU/ml) secured and tested.
  • Cytotoxicity grade 0.
  • A Regulatory Support File (RSF) is available for Ficoll-Paque PREMIUM products.

Ficoll-Paque PREMIUM products are based on Ficoll-Paque PLUS (see Ficoll-Paque PLUS) which has been used for more than 30 yr as a sterile density medium for the isolation of high yields of mononuclear cells from bone marrow, peripheral blood, and cord blood. All Ficoll-Paque PREMIUM products differ from Ficoll-Paque PLUS in that they are manufactured under a Quality Management System certified to ISO 13485:2012.

Applications

Ficoll-Paque PREMIUM
Optimized for the isolation of mononuclear cells from human peripheral blood, bone marrow, and umbilical cord blood.

Ficoll-Paque PREMIUM 1.084

Can be used for isolating a broad range of human mononuclear cells including those of a higher density and for separating blood cells from mice or rats.

Ficoll-Paque PREMIUM 1.073

Can be used when isolating lower-density human mononuclear cells (e.g., mesenchymal stromal cells or monocytes).

Ficoll-Paque PREMIUM

Application Optimized for the isolation of mononuclear cells from human peripheral blood, bone marrow, and umbilical cord blood.
Endotoxin Activity Max. < 0.12 EU/ml
Properties Ficoll-Paque PREMIUM products constitute a range of sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA in water for injection (WFI). The densities and osmolalities of the different products have been optimized for the isolation of mononuclear cells from blood and bone marrow
Estimated Shelf Life from Manufacture Date 3 years
Storage Conditions 4–30°C
Stability Ficoll-Paque PREMIUM products are provided as sterile solutions which are stable for at least 3 yr when stored between 4°C and 30°C and protected from light. Deterioration of Ficoll-Paque PREMIUM products is indicated by the appearance of a yellow color or particulate material in the solution
Physical State Liquid
Osmolality Max. 310 mOsm/kg
pH Range 5.5–7.5
Color Colorless to slightly yellow
Certificate of Analysis Yes
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