技术文章

Ficoll-Paque PLUS

17144002Ficoll-Paque PLUS 用于人**细胞的分离

Ficoll-Paque是从骨髓和血液分离人单核细胞的**试剂。Ficoll-Paque一致性性能和可重现性结果的坚实信誉是其在市场上销售30多年并被成千上万的同行评审文章所引用。

Ficoll-Paque产品是高分子量蔗糖聚合物和泛影酸钠溶液。Ficoll-Paque密度梯度介质利用简单快速离心程序从少量或大量血液中有效提取高产和高纯度的活单核细胞。Ficoll-Paque PLUS是无菌即用型产品,推荐用于单核细胞的常规分离。

2005年,GE医疗推出Ficoll Paque PREMIUM在质量管理系统下生产,符合 ISO 13485:2003标准。Ficoll-Paque PREMIUM设计用于制备人单核细胞。注:Ficoll-Paque被视为常规实验室试剂,并未归类为医疗设备。

主要特征

  • 经过严格的质量控制测试
  • 分离所得的细胞存活细胞率>90%
  • 低水平内**
  • 质量管理系统下生产,符合ISO 13485:2003标准(Ficoll-Paque PREMIUM)
  • 正确存储条件下稳定性至少保持3年

Ficoll-Paque PLUS

Ficoll-Paque Plus is a recognized standard in laboratories worldwide for the isolation of human lymphocytes forin vitro studies.

  • Ready to use sterile medium for isolation of human lymphocytes in high yield from peripheral blood.
  • Maintains viability and representative distribution of B and T lymphocytes.
  • Low levels of endotoxin (< 0.12 EU/ml).

Ficoll-Paque PLUS is a ready to use, sterile medium for isolation of lymphocytes in high yield from peripheral blood using a simple and rapid centrifugation procedure. It maintains the viability and a representative distribution of B and T lymphocytes.

Order Information For research use only.
Application For in vitro isolation of lymphocytes from human peripheral blood.
Density Max. 1.078 g/ml
Endotoxin Activity Max. < 0.12 EU/ml
Properties Ficoll-Paque PLUS is a sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA. The density has been optimized for the isolation of human lymphocytes from peripheral blood.
Estimated Shelf Life from Manufacture Date 3 years
Storage Conditions 4 - 30°C
Stability Ficoll-PaquePLUS is provided as sterile solution which is stable for at least 3 yr when stored between 4°C and 30°C and protected from light. Deterioration of Ficoll-Paque PLUS product is indicated by the appearance of a yellow color or particulate material in the solution
Physical State Liquid
pH Range 5.5 - 7.5
Color Colorless to slightly yellow
Certificate of Analysis Yes