超滤离心管

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产品名称: 超滤离心管

产品型号: UFC901096

产品展商: 密理博

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简单介绍

Millipore超滤管，15ml 10K

超滤离心管 的详细介绍

产品名称 :Millipore超滤管

品牌 :密理博

货号 :UFC901096

规格 :15ml 10K

Amicon Ultra-4* and -15* Centrifugal Filter Units are designed for in vitro diagnostic use and intended for concentrating serum, urine, cerebrospinal fluid and other body fluids prior to analysis.

The premier devices for concentrating 1 – 4mL or <15 mL

High recovery Ultracel regenerated cellulose membrane in a range of molecular weight cut-offs

High retentate recovery of >90%

Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 10–15 minutes)

Heat-sealed membrane minimizes downstream extractables

100% integrity tested for reliable performance

Convenient sample monitoring with translucent housing and volume gradations

Direct pipettor sample access eliminates processing step to recover concentrate

High concentration factors of 80–100X

The Amicon Ultra-4 and Amicon Ultra-15 with Ultracel-10 membrane are CE marked.

Applications

Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms

Purification of macromolecular components found in tissue culture extracts or cell lysates

Concentration of dilute or pre-purified proteins from column eluents

Desalting and buffer exchange

Performance data - Amicon Ultra-4 Centrifugal Filter Unit

Typical Spin Times to Filtrate Volume

Spin conditions: 4000 xg swinging bucket rotor at 25 ° C, 4 mL sample.
3K, 10K: Cytochrome c (0.25 mg/mL); 30K, 50K: BSA (1 mg/mL); 100K: IgG (1 mg/mL).

Typical Protein Recovery

Membrane
NMWL, kDa Protein Solute Protein Retentate
Volume, mL Protein Retentate
Recovery, %

10 Cytochrome c, 12.4 kDa
(0.25 mg/mL) 0.20 95

4 mL starting volume in a fixed angle rotor at 7,500 x g at 25 °C. Spin times: 3K (30 min); 10 and 30K (10 min); 50K (5 min); 100K (15 min).

Performance data - Amicon Ultra-15 Centrifugal Filter Unit

Typical Spin Times to Filtrate Volume

Spin conditions: 4000 xg swinging bucket rotor at 25° C, 15 mL sample.
3K, 10K: Cytochrome c (0.25 mg/mL); 30K, 50K: BSA (1 mg/mL); 100K: IgG (1 mg/mL).

Typical Protein Recovery

Membrane
NMWL, kDa Protein Solute Protein Retentate
Volume, mL Protein Retentate
Recovery, %

10 Cytochrome c, 12.4 kDa
(0.25 mg/mL) 0.24 93.03

15 mL starting volume in swinging bucket rotor at 4000 x g at 25 °C. Spin times: 3K (40 min); 10K and 100K (20 min); 30K (10 min); 50K (15 min).

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Membrane NMWL, kDa	Protein Solute	Protein Retentate Volume, mL	Protein Retentate Recovery, %
10	Cytochrome c, 12.4 kDa (0.25 mg/mL)	0.20	95
4 mL starting volume in a fixed angle rotor at 7,500 x g at 25 °C. Spin times: 3K (30 min); 10 and 30K (10 min); 50K (5 min); 100K (15 min).