描述 Thermo Scientific B-PER Bacterial Protein Extraction Reagent is a nonionic detergent-based solution that effectively disrupts cells and solubilizes native or recombinant proteins without denaturation. All B-PER Reagents are compatible with downstream applications, such as affinity chromatography (e.g., immobilized metal affinity chromatography, glutathione chromatography), SDS-PAGE, and protein quantification (e.g., Pierce BCA Protein Assay, Pierce 660nm Protein Assay). Depending on the particular application, protease inhibitors, salts, reducing agents, denaturants, and chelating agents may be added to the reagent.
• Detergent in Tris buffer; no enzyme components • Bacterial lysis • Purification of affinity tagged proteins
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